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. 2021 Mar 3;13(5):1079. doi: 10.3390/cancers13051079

Figure 3.

Figure 3

EphB6 regulates the crosstalk with lung epithelial cells. (A) Balloon plot summarizing GSEA results of AT1-like cells in monoculture and cocultured with D2.0R-EGFP-shCtrl cells or shEphB6 cells (results from two independent shEphB6 sequences, #31 and #35). Balloon size represents the statistical significance (−log10 FDR), while color indicates the fold-enrichment for each term (NES). Complete the list of gene sets in Figure S1. (B) Representative images of Ki67+ lung resident cells surrounding GFP+ disseminated indolent breast cancer cells with control shRNA or shRNAs targeting EphB6. Scale bar, 10 μm. (C) Quantification of images in B. Percentage of D2.0R-EGFP cells in contact with the indicated number of Ki67+ lung cells. n = 191, 143 and 177 cells for shCtrl, shEphB6#31 and shEphB6#35, respectively, across 3 mice/sample. P-value of the percentage of D2.0R-EGFP cells contacting 3 Ki67+ lung cells. 2 way ANOVA, multiple comparisons.