Fig. 5.
GMT of divergent B6 and PWD gut microbiota into genetically identical hosts modulates EAE. B6-GF mice were inoculated with cecal contents from B6 or PWD donors. (A) β-Diversity analysis by unweighted UniFrac distance of fecal microbiomes of B6 and PWD donors (collected prior to cecal content collection) and ex-GF recipients, collected at 1 wk or 4 wk posttransplant, as indicated. (B) EAE was induced and evaluated in the ex-GF B6 GMT recipients of B6 and PWD microbiomes at 4 wk posttransplantation, as described in Fig. 3. Mean daily clinical scores are shown, with overall significance determined by Friedman’s nonparametric two-way ANOVA as in Fig. 3. (C) Vertical transmission of B6 and PWD microbiomes in B6 hosts was established by using ex-GF recipients as founder breeding pairs. EAE was induced and evaluated as above. (D and E) Comparison of differential abundance of bacterial families (D) and genera (E) in ex-GF recipients of B6 vs. PWD microbiomes at 4 wk posttransplantation, prior to EAE induction. Families and genera exhibiting differential abundance as determined using a cutoff of log2(fold change) ≥ |1| and Pcorr < 0.05 are shown with all 16S data rarefied to 10,000 reads.