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. 2020 Oct 19;117(44):27516–27527. doi: 10.1073/pnas.2002817117

Fig. 7.

Fig. 7.

Partial transfer of gut microbiota from PWD to B6 mice by cohousing is sufficient to modulate EAE. Three-week-old B6 mice were randomized to be cohoused with age-matched PWD mice, or a different litter of age-matched B6 mice as controls. (A) Unweighted UniFrac distance analysis of fecal microbiomes before cohousing (B6 start and PWD start), and after 5 to 6 wk of cohousing (B6 control, B6 cohoused, PWD control, PWD cohoused). (B) EAE was induced and evaluated at 5 to 6 wk postcohousing, as described in Fig. 5. (C) Indicator species analysis was used to identify OTU distinguishing microbiomes of B6 control from B6 cohoused groups. Relative abundance of the identified OTUs, as calculated using DESeq2, is shown. (D) Random forest classifier analysis was used to identify OTUs distinguishing microbiomes of B6 control from B6 cohoused groups, and the relative importance/weight of these OTUs in the model is shown with all 16S data rarefied to 12,000 reads.