Fig. 7.

Evolution of ShK-like2 gene. (A) Distribution of the ShK-like2 homologs across the phylogenetic tree of sea anemones (adapted from ref. 41); the species with sequences genomes are in red. The number of alternative signal peptides for each ShK-like2 homolog identified in each genus (transcriptome or genome) is represented by ovals (orange, 2; blue, 1; gray, no homologs); the asterisk denotes that full-length sequences were obtained by joining shorter fragments manually. (B) Alignment of ShK-like2 homologs from different sea anemones. The alignment was built using Clustal Omega online tool with minor manual adjustments. Exon–intron structure is conserved between Nematostella, Actinia, and Exaiptasia (in red) and shown above the alignment. (C) Alignment of exon–intron structures of ShK-like1, ShK-like2, and ShK-like3 genes.