Fig. 3.

RNA localization at the invasive front requires microtubules. (A) Representative in situ hybridization images detecting RAB13 and RHOA RNAs within leader cells of invasive spheroids treated for 45 min with the indicated compounds. Associated probability density plots are derived from n = 28 to 29 images, two independent experiments. P values determined by the Wilcoxon signed-rank test. (Scale bar, 15 μm.) (B) Comparison of normalized RAB13 RNA distances. ****P < 0.0001; ns, nonsignificant by ANOVA with Dunnett’s multiple comparisons test. Error bars show SE.