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AfrLEA6 undergoes a liquid-liquid phase separation that sequesters GFP based on surface charge. (A) AfrLEA6 (0.17 mg/mL) separates from solution into a liquid phase in a buffer mimicking the intracellular milieu of A. franciscana. (B) Standard GFP (stGFP-7) is partitioned outside of the AfrLEA6 droplet. (C) Positive GFP (pGFP+36) is selectively partitioned and enriched within the droplet, and (D) highly natively charged GFP (nGFP-30) is also excluded.
