Fig. 5.

In vitro LLPS of AfrLEA6 is dependent on the SMP domain, while condensates fusion is facilitated by the predicted protein-binding domain. Kc167 cells expressing truncated versions of AfrLEA6 were imaged using confocal microscopy. Colocalization of GFP with or exclusion from AfrLEA6 condensates is visualized by comparing images that overlay red (mCherry) and green (GFP) fluorescence (Left) with images showing only the green fluorescence signal (Right). (A) LLPS of AfrLEA6 was absent when both SMP domains were removed. (B) LLPS of AfrLEA6 occurred when the predicted protein-binding domain was removed, but condensates fusion was hindered. (C) Removing only one of two SMP repeats had no apparent effect on the LLPS of AfrLEA6.