AfrLEA6 expression increases osmotic stress tolerance toward NaCl and sucrose. Nontransfected control (NTC), vector control (VC), and cells expressing AfrLEA6 were cultured for 48 h in culture medium supplemented with +400 mOsmol/kg of either NaCl (white bars) or sucrose (gray bars). In both cases, expression of AfrLEA6 significantly increased the osmotic stress tolerance of Kc167 cells. n = 9 to 18; P < 0.05, ANOVA. Different capital letters denote significant differences between cell lines in response to NaCl, while lowercase letters denote significant differences in response to sucrose.