Fig. 1.

CHK-124 and CHK-263 protect mice against CHIKV diseases and dissemination. (A–J) CHK-124 and CHK-263 mAbs reduced foot swelling and the viral RNA levels in ankles, muscles, blood, and spleen of CHIKV-infected wild-type (A–E) or congenic FcRγ^−/− (F–J) mice. Four-week-old wild-type or FcRγ^−/− C57BL/6 mice were treated with 100 µg of CHK-124, CHK-263, or an isotype control (WNV E60) 1 d before infection with 10³ FFU of CHIKV. (A and F) Foot swelling was measured at 3 dpi. Horizontal line indicates mean values (n = 5 to 10/group; two experiments; one-way ANOVA with a Tukey’s posttest; ****P < 0.0001). (B and G) Ipsilateral ankle, (C and H) serum, (D and I) spleen, and (E and J) contralateral ankle and gastrocnemius (calf) muscle were harvested 3 dpi, and viral RNA levels were determined by qRT-PCR. Bars indicate median values (n = 5 to 10/group); two experiments; Kruskal–Wallis with a Dunn’s posttest; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Each symbol in this figure represents data from an individual mouse.