Table 2. Summary of the experimental conditions and determined affinities in Figure 3.

Figure 31	Probe ligand	Target	Exptl data	[Cu]tot (µM)	[L]tot (µM)	p[Cu+] at2		logKD(P)3
						Start	End
b	Fs	CopK	Solid dots	31.0	90	9.1	10.3	<−9
			Empty dots	15.5	45	8.8	10.0	<−9
c	Fs	CopK	Solid dots	30.0	280	10.9	11.7	−11.2
			Empty dots	15.0	140	10.6	11.4	−11.2
d	Fz	CopK	Solid dots	30.0	120	11.2	11.8	−11.2
			Empty dots	15.0	60	10.9	11.9	−11.2
f	Bca	hGrx1	Solid dots	34.0	200	13.9	15.0	<−14
			Empty dots	34.0	100	12.7	14.5	<−13
g	Bca	hGrx1	Solid dots	38.0	500	14.9	15.4	−15.5
			Empty dots	19.0	250	14.6	15.2	−15.5
h	Bcs	hGrx1	Solid dots	32.0	80	14.7	15.8	−15.6
j	Bca	Atx1	Triangles	40	500	14.8	15.8	<−16
			Pluses	40	100	12.1	14.4
		WNL5–6	Circles	40	500	14.8	15.8	<−16
			Crosses	40	100	12.1	14.3
k	Bcs	Atx1	Solid dots	36	500	17.5	18.0	−17.7
			Empty dots		300	17.0	17.9	−17.7
l	Bcs	WLN5–6	Solid dots	36	500	17.5	18.0	−17.6
			Empty dots		200	16.4	17.6	−17.6

¹In Mops or KPi buffer (pH 7.0) containing excess reductant NH₂OH and/or ascorbate;

²Calculated based on the reported β₂ Cu^IL₂ in refs. [8] and [33] (see Table 4);

³Derived from fits of the experimental data to equation 14a (for expts. c,d,g,h,k,l) based on reported β₂ for Cu^IL₂ given in refs. [8] and [33] (see Table 4).