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. 2021 Feb 26;12:599641. doi: 10.3389/fimmu.2021.599641

Figure 4.

Figure 4

The inhibition of intracellular mycobacterial growth mediated by CD4+CD161+ T cells involved with autophagy in infected hMDM. Bar graph (A) shows that autophagy was involved inCD4+CD161+ T cell-mediated inhibition of intracellular mycobacterial growth. BCG-infected hMDM were pre-treated for 2 h with DMSO or 3-MA (10 uM), respectively, and then washed. These BCG-infected hMDMs were individually co-cultured for 72 h with CD4+CD161+ T cells or CD4+CD161- controls at a ratio of 10:1. Data shown as mean ± SD are derived from five experiments involving 10 healthy donors. ***p < 0.001 (ANOVA, Tukey’s test). Graph (B) shows thatCD4+CD161+ T cell could increase the LC3B puncta in BCG-infected hMDM. BCG-infected hMDM were pre-treated for 2 h with 3-MA (10 uM), and then washed. Then, these BCG-infected hMDMs were co-cultured for 72 h withCD4+CD161+ T cells orCD4+CD161- T cells at a ratio of 10:1. White arrows indicate the LC3B puncta. Bar graph (C) shows the statistical analysis of LC3B puncta per cell in BCG-infected hMDM. LC3B puncta in more than 150 cells were counted in each group. Suppplementary Figure 1D shown the specificity of LC3B staining in hMDM. *p < 0.05, **p < 0.01, (ANOVA, Tukey’s test).