Skip to main content
. 2021 Feb 25;8:585691. doi: 10.3389/fcvm.2021.585691

Figure 1.

Figure 1

Validation of the RIP method to pull-down Ezh2-binding lncRNAs. (A) Representative immunoblots showing the expression of EZH2, GAPDH, H3K27me3, and H3 in mouse hearts treated with Angiotensin II (AngII) at 0 (Sham), 4 and 24 h. (B,C) Analyzed results of EZH2 expression in relative to GAPDH (B) and H3K27me3 expression in relative to H3 (C). Data are mean ± SEM; *P < 0.05 vs. sham; n = 3. (D–G) Relative expression of known EZH2-binding lncRNAs including Chaer (D), Hotair (E), Mirt1 (F), and H19 (G) in mouse hearts treated with Angiotensin II (AngII) at 0 (Sham), 4 and 24 h. Data are mean ± SD from two independent experiments.