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. 2021 Feb 25;8:585691. doi: 10.3389/fcvm.2021.585691

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Copyright © 2021 Wang, Guo, Li, He, Zheng, Li and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the interaction between EZH2 and Snora33. (A) Predicted interaction strength between EZH2 and Snora33 using catRAPID. Among a thousand of random RNA sequences, Snora33 ranked 97% propensity to interact with EZH2. (B) RNA pull-down analysis to confirm the direct interaction between Snora33 and EZH2. Biotin was conjugated to the 3′ of Snora33 RNA in vitro, followed by streptavidin pull-down assay. Immunoblotting was performed with anti-EZH2 antibody using the elute, flow-through and input samples.