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. 2021 Feb 24;12:607572. doi: 10.3389/fphar.2021.607572

FIGURE 6.

FIGURE 6

Astrocytes activation: GFAP immunohistochemistry in spinal cord. Sections of dorsal horn of spinal cord (images A-H, i) processed for the immunohistochemistry of glial fibrillary acid protein (GFAP) and densitometric analysis (ii) of the mean immunoreaction area in both dorsal and ventral horns. In all panels, treatments are indicated as follows: (A) SHAM WKY + saline solution; (B) SHAM SHR + saline solution; (C) CCI SHR + saline solution; (D) CCI SHR + (+/−)-thioctic acid 250 μmol·kg−1·die−1; (E) CCI SHR + (+/−)-thioctic acid 125 μmol·kg−1·die−1; (F) CCI SHR + (+)-thioctic acid 125 μmol·kg−1·die−1; (G) CCI SHR + (−)-thioctic acid 125 μmol·kg−1·die−1; (H) CCI SHR + Pregabalin 300 μmol·kg−1·die−1. Calibration bar: 25 μm. Data are expressed as mean ± SEM; *p < 0.05 vs SHAM SHR + saline solution; ^p < 0.05 vs CCI SHR + saline solution.