Figure 1.

Effects of the engineered strain of MG1363-pMG36e-GLP-1 (M-GLP-1) on body weight in high fat diet mice. (A) Expressions of GLP-1 in bacteria. The Lactococcus lactis MG1363 were transformed with prokaryotic expression vector pMG36e-GLP-1 or pMG36e (control) and the fermentation supernatant were collected for detection of the GLP-1 levels using an Elisa Kit after the bacteria were cultured overnight. (B) Experimental schematic in mice. The hyperglycemia/obesity mouse model was induced by high fat diet (HFD) for evaluating the therapeutic effects of the engineered strain of M-GLP-1. The mice were given PBS or M-GLP-1 after 15 weeks of HFD feeding, respectively. (C) The curves of bodyweights in the mice. The HFD mice were fed with HFD for 15 weeks and then divided into two groups at 15^th week for treated with PBS or GLP-1 for 1 month, respectively. (D) Percentage of bodyweight gain in mice. The bodyweights of the PBS and GLP-1 treated mice which were fed with HFD were measured every other day, and the percentage of the bodyweight gain was represented with the initial bodyweight of the mice at 15^th week HFD fed as a control. The values represent the means ± SD, *p < 0.05, and ***p < 0.001, n = 5 per group.