TABLE 2.

Protein identification in the active gel lanes by LC-MSMS.

Band gel lane	Protein ID	Score	Number of identified peptides	Protein sequence coverage (%)	Genome annotation
Lower gel band lane	323309	295	10	19	GH32 family/GH116 family
	371227	204	8	16	GH32 family
	376719	166	5	9	Multicopper oxidase
	327740	165	6	16	GH43 family
	383312	149	7	14	Glucooligosaccharide oxidase
	387674	122	6	10	GH64 family
	383083	120	5	16	GH17 family
	417764	111	4	7	GH32 family
	381496	62	5	11	Putative oxidoreductase
Upper gel band lane	384244	423	20	54	GMC oxidoreductase family
	315441	274	12	42	GH2 family
	400960	253	11	32	Uncharacterized protein
	371227	185	10	34	GH32 family
	385992	181	10	32	Amidase
	323309	177	12	33	GH32 family/GH116 family
	401322	99	7	18	GH20 family
	373946	147	11	28	GH3 family
	383654	145	11	31	Uncharacterized protein
	401322	99	7	18	GH20 family

Protein bands were in situ digested and analyzed by LC-MSMS, and raw data was searched on the protein database as described in details in the “Materials and Methods” section. Search details and extensive description of the identified peptides are reported in Supplementary Material 2. The proteins belonging to the Gh32 family are highlighted in bold. Description of the glycosyl hydrolase (GH) families according to CAZypedia (http://www.cazypedia.org/): GH2: includes the following activities, β-galactosidases, β-glucuronidases, β-mannosidases, exo-β-glucosaminidases, and a mannosylglycoprotein endo-β-mannosidase; GH3: groups together exo-acting β-D-glucosidases, α-L-arabinofuranosidases, β-D-xylopyranosidases, N-acetyl-β-D-glucosaminidases, and N-acetyl-β-D-glucosaminide phosphorylases; GH17: includes two major groups of enzymes with related but distinct substrate specificities, namely, 1,3-β-D-glucan endohydrolases and 1,3;1,4-β-D-glucan endohydrolases; GH20: includes exo-acting β-N-acetylglucosaminidases, β-N-acetylgalactosamindase β-6-SO₃-N-acetylglucosaminidases, and exo-acting lacto-N-biosidases; GH32: includes: enzymes able to hydrolyze fructose containing polysaccharides, i.e., inulinases, invertases, levanases, and other; GH43: includes the following major activities, α-L-arabinofuranosidases, endo-α-L-arabinanases (or endo-processive arabinanases) and β-D-xylosidases; GH64: laminaripentaose-producing β (1,3)-glucanases; GH116: includes enzymes with diverse specificities, including β-xylosidase, β-glucosidase, and N-acetylglucosaminidase; GMC oxidoreductase: glucose dehydrogenase/choline dehydrogenase/mandelonitrile lyase.