TABLE 3.

Enzymatic inulin conversion under the experimental conditions (pH, T, substrate concentration, and enzyme loading) explored in the CCRD.

	pH	°C	Substrate concentration (g L–1)	U gsubstrate–1	Fructose (g L–1)	St. dev.	Conversion efficiency %
1	5	37.5	45	27.5	39.66	0.79	73.4
2	5	37.5	45	42.5	44.06	0.64	81.6
3	5	37.5	55	27.5	45.05	1.01	68.3
4	5	37.5	55	42.5	53.23	1.07	80.7
5	5	52.5	45	27.5	36.03	0.98	66.7
6	5	52.5	45	42.5	41.24	0.50	76.4
7	5	52.5	55	27.5	47.26	0.51	71.6
8	5	52.5	55	42.5	52.81	0.24	80.0
9	7	37.5	45	27.5	35.61	0.25	65.9
10	7	37.5	45	42.5	41.95	0.62	77.7
11	7	37.5	55	27.5	37.87	0.21	57.4
12	7	37.5	55	42.5	47.70	0.23	72.3
13	7	52.5	45	27.5	24.72	0.61	45.8
14	7	52.5	45	42.5	32.77	0.08	60.7
15	7	52.5	55	27.5	35.44	1.08	53.7
16	7	52.5	55	42.5	40.17	0.77	60.9
17	4	45	50	35	45.05	0.36	75.1
18	8	45	50	35	26.36	1.07	43.9
19	6	30	50	35	35.01	1.38	58.3
20	6	60	50	35	22.99	0.01	38.3
21	6	45	40	35	42.14	1.26	87.8
22	6	45	60	35	60.00	1.08	83.3
23	6	45	50	20	43.26	0.21	72.1
24	6	45	50	50	53.01	0.59	88.3
25	6	45	50	35	47.07	1.02	78.4
26	6	45	50	35	46.03	0.42	76.7

Fructose concentration has been determined after 24 h incubation. Conversion efficiency has been calculated as reported in the section “Enzymatic Hydrolysis of Inulin”.