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. 2021 Feb 24;12:615102. doi: 10.3389/fimmu.2021.615102

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Copyright © 2021 Fahad, Timm, Madan, Burgomaster, Dowd, Normandin, Gutiérrez-González, Pennington, De Souza, Henry, Laboune, Wang, Ambrozak, Gordon, Douek, Ledgerwood, Graham, Castilho, Pierson, Mascola and DeKosky

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Repertoire-scale functional data for mAb lineages targeting flavivirus antigens. (A) Computational interrogation of yeast display NGS data after 2 rounds of sorting revealed the functional profile of anti-flavivirus antibodies in convalescent donors. Antibodies were screened against three antigens: ZIKV wild-type VLPs [ZIKV (wt)], ZIKV immature VLPs [ZIKV (pr-mut)], and YFV wild-type VLPs [YFV (wt)]. NGS data from Round 2 was used to calculate enrichment ratio and determine antigen specificity while antibody affinity was predicated based on enrichment in Round 3 affinity gates. Antibody isotype was determined from natively paired heavy:light sequence data. (B) Number of isolated mAb lineages and their antigen reactivity profiles for each donor.