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. 2021 Mar 8;2021:5597394. doi: 10.1155/2021/5597394

Figure 4.

Figure 4

The effects of miR-155 silencing on podocyte inflammation was reversed by SIRT1 downregulation. (a, b) SIRT1 expression in podocytes transfected with the miR-155 mimic or inhibitor under high glucose conditions was evaluated using real-time PCR and Western blotting analysis. #Compared with control group, P < 0.05; ∗Compared with HG (high glucose) group, P < 0.05. (c, d) The expression of SIRT1 in podocytes transfected with the miR-155 inhibitor and SIRT1 siRNA under high glucose conditions was assessed using real-time PCR and Western blotting analysis. #Compared with HG (high glucose) group, P < 0.05; ∗Compared with miR-155 inhibitor + HG group, P < 0.05, the same below. (e–h) Inflammatory cytokine expressions (MCP-1, TNF-α, IL-1β, and IL-6) in podocytes transfected with the miR-155 inhibitor and SIRT1 siRNA under high glucose conditions was determined using ELISA. (i) Nephrin expression in podocytes cotransfected with the miR-155 inhibitor and SIRT1 siRNA under high glucose conditions was quantified using Western blotting analysis.