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Method of ROI analysis. First, 10 points are manually placed on the contour of both the pituitary (PIT) and postsphenoid ossification, and a round ROI (diameter, 5 pixels) is drawn on both the pons and sphenoid bone. With the convex hull function of ImageJ software, these 10 points are automatically connected to provide an ROI defining the contours of both the pituitary gland and postsphenoid ossification.
