Figure 3.

Expression of miR-335-5p is significantly higher in SW620-exo than in SW80-exo

(A) Volcano plot of differentially expressed miRNAs in SW480-exo and SW620-exo. (B) Heatmap of differentially expressed miRNAs in SW480-exo and SW620-exo (fold change ≥ 5.0). (C) Quantitative RT-PCR analysis of miR-335-5p expression in SW480-exo and SW620-exo. Data are presented as mean ± SD (n = 3). ∗∗p < 0.01. (D) Expression of miR-335-5p in normal and CRC samples based on analysis of The Cancer Genome Atlas (TCGA) data. Data are presented as mean ± SD. p = 1.28e-08. (E) Survival analysis of CRC patients expressing high and low levels of miR-335-5p. p = 2.28e-01 (F) Gene-set enrichment analysis (GSEA) analysis showed that the differentially expressed genes associated with miR-335-5p level in CRC samples were enriched for the Ras protein signal transduction pathway. p < 0.001. (G) GSEA analysis showed that the differentially expressed genes associated with miR-335-5p level in CRC samples were enriched for the regulation of Ras protein signal transduction pathway. p = 0.004