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. 2021 Feb 24;21:14–27. doi: 10.1016/j.omtm.2021.02.016

Figure 2.

Figure 2

Effects of γ- and electron-irradiation on transcriptional regulation of PBMCs

(A) PCA of freshly isolated PBMCs, PBMCs after 24 h of culture, and PBMCs 24 h after 60 Gy γ- and 60 Gy electron-irradiation. Each dot represents one donor; color codes indicate treatments. (B) Number of differentially expressed genes among groups. (C) Scatterplots showing up- and downregulated genes (red and green, respectively) between non-irradiated and irradiated PBMCs. Each dot represents one gene. Gray dots indicate non-differentially expressed genes (fold-change cut-offs 2 and 0.5, respectively). CCL20, CC-chemokine ligand 20; CD101, cluster of differentiation 101; CSF1R, colony-stimulating factor 1 receptor; FPR3, formyl peptide receptor 3; GZMA, granzyme A; TRGV9, T cell receptor gamma variable 9. (D) Heatmaps of global gene expressions of irradiated and non-irradiated samples. Each row represents one gene; each column represents one donor with the respective treatment. Color codes indicate expression levels. (E) Differentially expressed genes between irradiated and non-irradiated cells were used to calculate associated signaling pathways. Circle sizes reflect total numbers of regulated genes per pathway, and p values are indicated by color code. Data of n = 4 donors are shown.