Figure 5.

Nanoparticle measurements of EVs secreted by γ- and electron-irradiated PBMCs

(A) Averaged concentrations per sizes of detected nanoparticles in medium and in secretomes of irradiated PBMCs. Pooled secretomes of n = 4 donors were analyzed. Black lines indicate mean of 6 measurements. Red areas indicate standard errors of the mean. (B and C) Statistical analyses of particle (B) concentrations and (C) sizes. Bar diagrams show mean and standard deviation. For statistical analysis, one-way ANOVA with Tukey’s multiple comparisons was performed. (D) Venn diagram showing the amount of identical and unique microRNAs detected in the EVs of γ- and electron-irradiated PBMCs. Absolute numbers indicate the number of different microRNAs, and percentages indicate the relative amounts of identical and unique microRNAs. (E) Heatmap of the most abundant microRNAs in the EVs of γ- and electron-irradiated PBMCs. Color code indicates unique molecular identifiers (UMIs).