Figure 6.

PBMCsec and secretomes of electron-irradiated PBMCs display comparable biological activity

(A) Representative micrographs of HUVECs incubated with medium or secretomes of irradiated PBMCs. Pooled secretomes of n = 4 donors were added. Scale bar, 250 μm. (B) Statistical analyses of nodes per field, junctions per field, and total segment lengths per field. Bar diagrams show arithmetic mean and standard deviation. Groups were compared using one-way ANOVA and Tukey’s multiple comparisons. (C) Representative images of murine, thoracic,aortic rings incubated with medium or secretomes of irradiated PBMCs. Pooled secretomes of n = 4 donors were added. Scale bar, 500 μm. (D) Statistical analysis of outgrowth areas. Boxplot diagrams were generated using first and third quartiles as boxes and median as bars. Whiskers indicate 10−90 percentiles. Groups were compared using one-way ANOVA and Sidak’s multiple comparisons. (E) AP-1 activation and (F) HSP27 phosphorylation induced by the secretomes obtained from irradiated PBMCs. Values were normalized to internally established reference standards. Bar diagrams show arithmetic mean and standard deviation. Groups were compared using one-way ANOVA and Tukey’s multiple comparisons.