Figure 3.

Putative Ari-1 substrates. Volcano plot showing differentially ubiquitinated proteins between ari-1^OE and control flies. Abundance of each individual protein was determined by the sum of LFQ intensities of the three replicas performed. LFQ ari-1^OE/Control ratios in the x-axis (in log₂ scale) and the t test p-values in the y-axis (in −log₁₀ scale) are displayed. The horizontal gray lane indicates the statistical significance (p-value < 0.05), while vertical gray lanes represent a twofold increase (log₂ LFQ ari-1^OE/Control = 1) or a twofold decrease (log₂ LFQ ari-1^OE/Control = −1), of the ubiquitination levels. Endogenously ubiquitinated proteins (ACC and PCB), Avidin (AVD), as well as ubiquitin (Ub) are shown in blue. Proteins whose ubiquitination increases or reduces at least twofold upon Ari-1 overexpression are shown in green and red, respectively. Ari-1 is shown in purple.