Table 1.
Solutions and buffers
| Solutions stored 21°C–25°C | Needed for Method 1 | Needed for Method 2 |
|---|---|---|
| Buffer A (recipe below) | ✓ | ✓ |
| Buffer B (recipe below) | ✓ | ✓ |
| Buffer C (recipe below) | ✓ | ✓ |
| 60% Buffer B (recipe below) | ✓ | ✓ |
| Trypsin Digestion Buffer (recipe below) | ✓ | ✓ |
| 70% ethanol | N/A | ✓ |
| 2% DMSO | N/A | ✓ |
| 10% TFE in Trypsin Digestion Buffer | N/A | ✓ |
| 0.5 M TCEP | ✓ | ✓ |
| Aliquoted frozen stock solutions | ||
| 1 M DTT in LC/MS-grade water (store −20°C) | ✓ | ✓ |
| 550 mM 2-iodoacetamide in LC/MS-grade water (store −20°C, protect from light) | ✓ | ✓ |
| Trypsin 1 μg/10 μL in 10 mM acetic acid (store −80°C) | ✓ | ✓ |