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Solution preparations
| Reagent | Final concentration LC/MS-grade water | Final concentration LC/MS-grade Acetonitrile | Final concentration Formic Acid |
|---|---|---|---|
| Buffer A | 100% | 0% | 0.1% |
| Buffer B | 0% | 100% | 0.1% |
| Buffer C | 95% | 5% | 0.1% |
| 60% B | 40% | 60% | 0.1% |
| Trypsin digestion buffer | Final concentration |
|---|---|
| Tris-HCL pH 8.0 | 50 mM |
| CaCl2 | 2 mM |
