Table 2.

Filtering criteria for putative DNMs

Cases	Controls
•Minor allele frequency ≤ 4 × 10−4 across all samples in 1000 Genomes, EVS, and ExAC, Bravo	•in-cohort allele frequency ≤ 4 × 10−4
•probands must have at least 10 total reads and 5 alternative allele reads •probands must have a minimum of 20% alternate allele ratio for alternate allele reads ≥10 or a minimum 28% alternate ratio for alternate allele reads <10 •parents must have a minimum depth of 10 reference reads and alternate allele ratio <3.5% •exonic or canonical splice-site variants

Example filtering criteria to analyze DNMs in cases and controls. The criteria are the same as those used by Dong et al. (2020).