Skip to main content
. 2021 Feb 7;296:100396. doi: 10.1016/j.jbc.2021.100396

Figure 4.

Figure 4

USP11 is required for cell survival upon DNA damage by DPC-inducing agents.A, WB showing knockdown efficiency of USP11 in U2OS cells stably expressing nonsilencing shRNA control or two different shRNA sequences (sh1 and sh2) targeted to USP11. B–F, clonogenic cell survival curves for shRNA control and USP11 knockdown U2OS treated with indicated concentrations of (B) CPT for 24 h, (C) VP16 for 4 h, (D) formaldehyde for 20 min, (E) HU for 24 h, or (F) MMC for 24 h. G, WB showing knockdown efficiency of USP11 and SPRTN. H and I, clonogenic cell survival curves for USP11 and SPRTN single and double -knockdown cells treated with indicated concentrations of (H) CPT for 24 h or (I) VP16 for 2 h. B–F, H and I, percent cell survival was calculated. Data are presented as mean ± SD (n = 3). Statistical analysis: Two-tailed paired t-test was performed using confidence interval =90% and α = 0.1; ∗p ≤ 0.1, ∗∗p ≤ 0.05, ∗∗∗p ≤ 0.01. WB, western blot. See also Fig. S3.