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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Invest Dermatol. 2020 Sep 16;141(4):810–820.e8. doi: 10.1016/j.jid.2020.09.001

Figure 6. Antibody-mediated toxin neutralization protected against dermonecrosis.

Figure 6.

(a) Bacteria were opsonized by mice serum and incubated with mouse or human neutrophils for bacterial killing, and bacterial survival was quantified. (b) Mice serum was incubated with Hla before incubation with mouse or human neutrophils. Neutrophil viability was quantified. (c) Mice serum was incubated with 1 μM Hla before incubation with mouse or human monocytes from the peripheral blood. (d) Mice received a passive transfer of naive serum, IgG, or Fabs before SSTI. (n = 4–5). Data were compared using (a, c) one-way ANOVA with Tukey’s test, (b) Kruskal–Wallis test with Dunn’s post-hoc test, or (d) two-way ANOVA with repeated measurements with Tukey’s test. Results in a–c were a compilation of 2–5 independent experiments using 5–15 mice in each experiment and blood from a total of six different healthy human donors. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***p < 0.001, ****P < 0.0001. Hash (#) indicates the number of cells per lesion. Fab, antigen-binding fragment; Hla, α-hemolysin; NS, not significant; SSTI, skin and soft tissue infection.