Table 2.
MSC isolation and characterisation
| Author | MSC source | Method of tissue extraction | MSC characterisation | MSC treatment |
|---|---|---|---|---|
| Hu et al. (2019) | Murine bone marrow | Needle aspiration from tibia and femur | Flow cytometry (CD150+/CD74+), tri-lineage differentiation (osteogenic, adipogenic, chondrogenic) | Passages 8–13 harvested at 70% |
| Hu et al. (2019) | Murine bone marrow | Needle aspiration from tibia and femur | Flow cytometry (CD105 + /CD73+), tri-lineage differentiation (osteogenic, adipogenic, hepatogenic) | Passages 8–13 harvested at 70% |
| Liu et al. (2018) | Human adipose | Surgical excision of redundant tissue from surgical operations | Flow cytometry (CD105+/CD90+/CD34-/CD45-/CD19-), adipogenic and osteogenic differentiation | Passages 3–4 |
| Foubert et al. (2017) | Porcine adipose | Surgical excision of inguinal fat pad | Flow cytometry (CD90+/CD45-) | Untreated, re-suspended in buffer solution and delivered two hours following isolation |
| Yates et al. (2017) | Human bone marrow | Immortalised cell line | Flow cytometry (CD105+/CD14-/CD34-/CD45-), tri-lineage differentiation (osteogenic, adipogenic, chondrogenic) | Passage 4 harvested at 70% |
| Yates et al. (2017) | Human bone marrow | Needle aspiration from posterior iliac crest | Flow cytometry (CD105+/CD14-/CD34-/CD45-), tri-lineage differentiation (osteogenic, adipogenic, chondrogenic) | Passage 3 harvested at 70% |
| Domergue et al. (2016) | Human adipose | Dermolipectomy | Flow cytometry (CD73+/CD90+/CD34-/CD14-) | End of passage 1 |
| Li et al. (2016) | Human adipose | Liposuction | Flow cytometry (CD73+/CD90+/CD34-/CD14-), adipogenic and osteogenic differentiation | Passages 3–5 starved for 24 h at 80–90% confluence prior to supernatant collection |
| Zhang et al. (2015) | Rabbit adipose | Surgical excision of inguinal fat pad | Flow cytometry (CD73+/CD90+/CD34-/CD14-), adipogenic and osteogenic differentiation | Passage 3 harvested at 80–90% |
| Liu et al. (2014) | Human bone marrow and rabbit bone marrow | Human: bone marrow biopsy Rabbit: bone marrow needle aspiration | Flow cytometry (CD105+/CD90+/CD34-/CD45-), chondrogenic and osteogenic differentiation | Passage 3–4 |
| Liu et al. (2014) | Rabbit bone marrow | Needle aspiration from tibia and femur | Flow cytometry (CD105 + /CD90 + /CD34-/CD45-), adipogenic and osteogenic differentiation | Passage 4–6 |