Fig. 2.

Pregnancy hormones drive changes to the active regulatory landscape of pre-pregnancy mammary organoid cultures. (a) RNAseq quantification of parity gene signature in pre-pregnancy organoids treated with complete medium for 9 days. n = 2 biological replicates. (b) GSEA of gene networks differentially expressed in pre-pregnancy organoids treated with complete medium for 9 days. (c) Venn diagrams comparing H3K27ac peaks of pre-pregnant mammary organoid cultures before complete medium treatment (0 h) and after 3 h, 12 h and 48 h treatment with complete medium. (d) Density plot showing H3K27ac levels at computationally defined DNA binding motifs recognized by the transcription factors ESRa, MYC and STAT5 in pre-pregnancy mammary organoids before complete medium treatment (0 h) and after 3 h, 12 h, 48 h treatment with complete medium