Fig. 1.

Localization of integrin α11β1 in cells spreading on collagens XIII and I. C2C12 cells transfected with integrin α11-EGFP were seeded onto coverslips coated with collagen XIII (Col XIII) ectodomain a and collagen I (Col I) c and allowed to adhere at 37 °C for 2 h. The cells were then fixed and imaged on a fluorescence microscope with direct detection of EGFP-tagged protein. e The C2C12-α11+ cells spreading on the fibronectin-coated surface. Primary wild type MEFs spread on collagen XIII b and on collagen I d under the same conditions as the C2C12-α11+ cells and were visualized with indirect immune fluorescence staining using an antibody against the cytoplasmic domain of mouse integrin α11. e C2C12 cells were stably transfected with integrin α11, and the expression of α11 was analyzed by western blotting using an antibody against the α11 cytoplasmic domain