Fig. 2.

Adhesion of integrin α11-transfected C2C12 cells to fibronectin and collagens (a) C2C12 control cells and cells transfected with an integrin α11cDNA (C2C12-α11+) were seeded on a plastic surface coated with fibronectin, collagen I, or the collagen XIII ectodomain. The cells were analyzed quantitatively using the β-hexosaminidase release assay for the attachment to fibronectin, collagen I, and collagen XIII. Data are presented relative to fibronectin and BSA binding, where the saturated binding to fibronectin represents 100% and the background binding to a BSA-coated surface represents 0%. Values are shown as means of triplicates with standard deviation bars. (b, b’) Microscopic monitoring of the attachment and morphology of C2C12 and C2C12-α11 cells adhering on collagen XIII. Scale bars 200 μm