Skip to main content
. 2021 Mar 2;11:623769. doi: 10.3389/fphys.2020.623769

FIGURE 5.

FIGURE 5

VEGFR2 mutation impaired cell orientation and cell polarity in vivo. (A) Representative images of VE-cadherin staining of endothelial cell from the aortas of littermate P6 pups (CTR) or carrying VEGFR2 mutation (Y949F) (scale bars: 25 and 10 μm, respectively). (B) Quantification of cells aligned with the flow direction (in between 15° around the flow axe, N = 5, Data presented as Mean + SEM. (C) Representative images of golgi staining of endothelial cell from the aortas of P6 pups littermate (CTR) or carrying VEGFR2 mutation (Y949F) (scale bars: 25 μm). (D) Quantification of golgi position around the nucleus compared to the flow direction (N = 5; with: in between 0 and 30° around the flow axe, side: 30–150°, against: 150–180°). Two-way ANOVA; Tukey’s post hoc, *p < 0.05; ***p < 0.001.