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Efficacy of free cyclophosphamide combined with the hydrogel cancer vaccine. a) Photographs of tumors in different groups. b) Bodyweight curves of CT26 tumor-bearing mice over time, the black arrows represent CTX administration and the red arrows represent hydrogel vaccine administration. c) Tumor weight in all groups. d) Tumor growth curves of different treatment groups. e) Serum cytokine levels in CT26 tumor-bearing mice (n = 3–4). *p < 0.05, **p < 0.01 and ***p < 0.001.
