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Immune cell activation of free cyclophosphamide combined with the hydrogel cancer vaccine. a) Representative flow cytometric analysis images. b-d) Relative quantification of CD3+, CD4+, and CD8+ T cells in inguinal lymph nodes. e-g) Relative quantification of CD11c+, CD86+, and MHC-I + DCs in inguinal lymph nodes analyzed by flow cytometry. *p < 0.05, **p < 0.01 and ***p < 0.001.
