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. 2021 Mar 15;12(3):270. doi: 10.1038/s41419-021-03565-3

Fig. 4. Hsa_circ_0124554 active AKT through directly binding.

Fig. 4

a RNA pull-down assay was conducted in cells with hsa_circ_0124554 sense and antisense. b Western blot analysis with anti-AKT antibody indicated the existence of AKT within the hsa_circ_0124554 sense RNA probe pull-down samples in HT 29 and HCT 116 cells. c RIP assay was performed using an anti-AKT antibody and was confirmed with agarose gel electrophoresis using a different probe. d Fold increases of RIP were calculated by comparison with the input. e Protein expression of AKT in cells with hsa_circ_0124554 overexpression or suppression. Data are presented as means ± SD (**P < 0.01).