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. 2021 Mar 15;11:5923. doi: 10.1038/s41598-021-85148-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Panel (a) Gating strategy of circulating monocyte and dendritic cell (DC) subsets by multiparameter flow cytometry. Cells that were negative for CD3, CD19 and CD20 (lineage negative) and HLA-DR⁺ were gated (R1). CD14 and CD16 negative cells (DCs) were then separated from monocytes. Classification of classical (CD14⁺ CD16^neg), intermediate (CD14⁺ CD16⁺) and nonclassical monocytes (CD14^lo CD16⁺). DCs were classified according to CD11c and CD123 expression in classical (cDC) (CD11c⁺ CD123^lo) and plasmacytoid DCs (pDC) (CD123⁺⁺ CD11c^neg). Panel (b) Monocyte and DC clusters identified by SPADE. Cellular phenotypes were assigned to the SPADE tree, based on node expression and location. cDC classical dendritic cell, cMo Classical monocytes, DCs Dendritic cells, iMo Intermediate monocytes, ncMo Nonclassical monocytes, pDC Plasmacytoid dendritic cell, TiMas Tissular macrophages (iMo + ncMo).