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. 2021 Mar 15;11:6052. doi: 10.1038/s41598-021-85606-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of type 2 diabetes progression on endothelial function in aorta and pulmonary artery (PA). Typical trace of a dose response relaxation curve to different concentrations of ACh (0.03–30 µM) in the aorta (a) and pulmonary artery (b). Average responses in the aorta (c) and pulmonary artery (d) of the dose response relaxations in control (n = 10–14), HF (n = 10–11) and HFHSu (n = 10) animals. Values are presented as mean ± SD. Curves compared using two-way ANOVA with Dunnet’s Multiple Comparison test. *p < 0.05 and **p < 0.01 versus control animals; ^#p < 0.05 and ^##p < 0.01, HF versus HFHSu animals.