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. 2021 Mar 15;11:5887. doi: 10.1038/s41598-021-85327-x

Figure 6.

Figure 6

Impact of Petri dish size (90 mm vs 100 mm on the x-axis) on the total number of collected bacteria for the different colony-counting methods. The total number of collected bacteria was calculated on the six stages of the ACI for positive BFE runs (n = 4 for two different bacterial suspensions; 8 nebulizations). White bars: 90 mm petri dishes. Grey bars: 100 mm petri dishes. Method 1 corresponds to direct colony counting using an HD counter (2111 ± 310 counts for 90-mm dishes; 2781 ± 460 counts for 100-mm dishes). Method 2 corresponds to direct colony counting using an HD counter and then conversion into viable particles (5299 ± 916 counts for 90-mm dishes; 7659 ± 1177 counts for 100-mm dishes). Method 3 corresponds to visual colony counting of positive holes and then conversion (1809 ± 329 counts for 90-mm dishes; 2638 ± 480 counts for 100-mm dishes). Method 4 was used as a reference, corresponding to an approximation of the total airborne bacteria emitted by the bioaerosol nebulizer (3222 ± 1323 counts for 90-mm dishes; 3014 ± 1144 counts for 100-mm dishes). ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001.