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. 2021 Mar 15;11:5887. doi: 10.1038/s41598-021-85327-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of colony-counting methods on bioaerosol aerodynamic size (n = 2 for two different bacterial suspensions; 4 nebulizations/90-mm Petri dish). (A) Refers to the Median Aerodynamic Diameter (MAD). (B) Refers to the Mean Particle Size (MPS). Method 1 corresponds to direct colony counting using an HD counter (4.04 ± 0.16 µm for MAD; 3.70 ± 0.26 µm for MPS). Method 2 corresponds to direct colony counting using an HD counter and then conversion into viable particles (3.60 ± 0.22 µm for MAD; 3.11 ± 0.11 µm for MPS). Method 3 corresponds to visual colony counting of positive holes and then conversion (3.55 ± 0.08 µm for MAD; 3.33 ± 0.32 µm for MPS). ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001.