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. 2021 Mar 15;11:5887. doi: 10.1038/s41598-021-85327-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Aerosol size distribution (n = 2 for two different bacterial suspensions; 4 nebulizations/90-mm Petri dish). Method 1 (open circles) corresponds to direct colony counting using an HD counter. Method 2 (closed squares) corresponds to direct colony counting using HD counter and then conversion into viable particles. Method 3 (closed hexgons) corresponds to visual colony counting of positive holes and then conversion.