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. 2021 Mar 15;7:53. doi: 10.1038/s41420-021-00438-8

Fig. 6. Sirt1-induced deacetylation of ERα and reduction of adiposity in mice.

Fig. 6

a Effects of Sirt1 overexpression on ERα acetylation in mice. Upper, acetylated ERα was analyzed by immunoprecipitation (IP) with an anti-acetylated lysine (anti-acLys) antibody that pulled down acetylated proteins, followed by immunoblot (IB) with an anti-ERα antibody. Normal rabbit IgG (non-specific IgG) was used as negative control. Middle, whole lysates (WL) were analyzed by IB with anti-ERα and anti-GAPDH antibodies. Lower, densitometric analyses were used to quantify acetylated and total ERα in control (Ctrl) and Sirt1 transgenic (S1tg) mice. *p < 0.05; n = 8. b Effects of Sirt1 activator nicotinamide mononucleotide (NMN, 100 µM) on ERα acetylation in 3T3L1 cells. Left-upper, acetylated ERα was analyzed by IP with an anti-ERα antibody that pulled down ERα protein, followed by IB with an anti-acetylated lysine (anti-acLys) antibody. Normal rabbit IgG (non-specific IgG) was used as the negative control. Left-lower, whole lysates (WL) were analyzed by IB with anti-ERα and anti-GAPDH antibodies. Right, densitometric analyses were used to quantify acetylated and total ERα in 3T3L1 cells. DI, differentiation inducer. *p < 0.05 and **p < 0.01 by comparing DI(−) NMN(−) with DI(+) NMN(−). #p < 0.05 and ##p < 0.01 by comparing DI(+) NMN(−) with DI(+) NMN(+). n = 8. c Sirt1-induced reduction of adiposity was greater in female than in male mice. ***p < 0.001; n = 6–10. d A schematic view of Sirt1 coordinating with ERα to regulate autophagy and adipogenesis (adiposity).