Fig. 5. PER1 mediated OSCC cell proliferation through the glycolytic pathway.

A Western blot results showed that the increase in the expression of HK2, PKM2, and LDHA was significantly reversed after 2-DG was added to RNAi-PER1-TSCCA cells. B–D After 2-DG was added to RNAi-PER1-TSCCA cells, the increases in glucose uptake, lactate production, and the enzymatic activity of HK, PK, and LDH were significantly reversed. E, F The CCK-8 assay and MTT assay showed that the increased proliferation of RNAi-PER1-TSCCA cells was significantly reversed after 2-DG addition. All data are from three independent experiments. The data are presented as the mean ± SD values (n ≥ 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.