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. 2021 Mar 15;12(3):276. doi: 10.1038/s41419-021-03563-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The Co-IP results showed that PER1 interacted with RACK1 and PI3K to form the PER1/RACK1/PI3K complex. B The amino acid sequences of wild-type and mutant PER1 (the blank box represents the amino acid sequence of the deletion mutant). A stands for alanine, L for leucine, S for serine, Y for tyrosine, and R for arginine. C The Co-IP and western blot results showed that, in OE-PER1-SCC15 cells, the abundance of the PER1/RACK1/PI3K complex was significantly increased but the intracellular protein levels of total PI3K and p-AKT were significantly decreased, while in Mut-PER1-SCC15 cells, the increase in the abundance of the PER1/RACK1/PI3K complex and the decreases in the intracellular protein levels of total PI3K and p-AKT were significantly reversed. D The CHX chase experiment showed that the half-life of the PI3K protein in OE-PER1-SCC15 cells was significantly decreased, while the decrease in the half-life of the PI3K protein was significantly reversed in Mut-PER1-SCC15 cells. All data are from three independent experiments. The data are presented as the mean ± SD values (n ≥ 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.