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. 2021 Mar 15;11:5925. doi: 10.1038/s41598-021-85124-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-κB) signaling pathway in renal tissue in control, cecal ligation and puncture (CLP), and CLP + green propolis (CLP + GP) group rats 24 h after the CLP or sham procedure. (A) Immunoblotting of TLR4 protein expression in renal tissue; (B) Immunoblotting of NF-κB protein expression in renal tissues; (C) Macrophage infiltration in renal tissues analyzed by immunohistochemistry. We analyzed 30 grid fields (0.087 mm² each) per kidney cortex, counting the number of infiltrating CD68 + cells in each field, on the basis of which we calculated the mean numbers in the renal cortical tubulointerstitium and the mean counts per kidney. *p < 0.05 versus control and CLP + GP; ***p < 0.001 versus control and CLP + GP; **p < 0.01 versus control and CLP + GP.