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. 2021 Mar 15;11:5925. doi: 10.1038/s41598-021-85124-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of green propolis on Toll-like receptor 4 (TLR4) protein expression, macrophage infiltration, and apoptosis in lung tissue in control, cecal ligation and puncture (CLP), and CLP + green propolis (CLP + GP) group rats 24 h after the CLP or sham procedure. (A) TLR4 expression in macrophage cells. A double-labeling technique was used for CD68 and TLR4 analyzed by immunofluorescence; (B) Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) + cells analyzed by immunofluorescence. *p < 0.05 versus control and CLP + GP.