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. 2021 Mar 15;9:17. doi: 10.1038/s41413-021-00141-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Comparative analysis of the heterogeneity of monocytes/macrophages. a Monocyte/macrophage population after merging five scRNA-seq datasets was visualized with umap plot. b Identification of the 4 subclusters of Monocyte/Macrophage population. c The expression of classic macrophage polarization markers in monocyte/macrophage population. d GO enrichment analysis of the biological functions of different subclusters. e Distribution of cells on umap plot split by tissue origins. f Flow cytometry analysis of the ratio of Cd86⁺ and Cd206⁺ macrophages in alveolar bone marrow and long bone marrow