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LC-ESI-MS spectra of the hinge region peptide from recombinant IgA1 produced in the presence of Nb-P4H silencing constructs (Nb-P4H1 RNAi and Nb-P4H10 RNAi). Recombinant IgA1 was expressed by infiltration of pPT2M-IgA1. (A) Unmodified peptide and additional peaks showing P4H activity. (B) A wider mass range shows the same samples carrying additional O-glycans in the form of arabinosylation. Asterisks denote impurities unrelated to the target molecules.
